Coding

Part:BBa_K1797015:Design

Designed by: iGEM15_Tsinghua   Group: iGEM15_Tsinghua   (2015-09-17)


dCas9-Long Linker (Gly-Gly-Ser-Gly)x3-Bxb1 gp35


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
    Illegal NheI site found at 4349
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
    Illegal BamHI site found at 4623
    Illegal XhoI site found at 4710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5262
    Illegal NgoMIV site found at 5349
    Illegal AgeI site found at 4399
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5457


Design Notes

No.


Source

No.

References